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KMID : 0364820230590010008
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Korean Journal of Microbiology
2023 Volume.59 No. 1 p.8 ~ p.15
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Dechloromonas hankyongensis sp. nov., isolated from wetland
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Kim Ji-Won
Jo Jung-Hun
Im Wan-Taek
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Abstract
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A Gram-stain-negative, rod-shaped, motile via polar flagellum, facultatively anaerobic, creamy, bacterium (designated XY25T) was isolated from wetland from Eco Park in Godeok-dong, Gangdong-gu, Seoul, Republic of Korea. On the basis of 16S rRNA gene sequencing, strain XY25T clustered with species of the Dechloromonas and appeared closely related to Dechloromonas agitata CKBT 98.49%, Dechloromonas hortensis MA-1T 98.15%, and Dechloromonas denitrificans ATCC BAA-841T 97.67%. The average nucleotide identity (ANI) calculated between strain XY25T and each of the three strains (Dechloromonas agitata CKBT, Dechloromonas hortensis MA-1T, and Dechloromonas denitrificans ATCC BAA-841T) were 83.7, 81.2, and 81.0 %. And the digital DNA-DNA hybridization (dDDH) calculated between strain XY25T and each of the three strains (Dechloromonas agitata CKBT, Dechloromonas hortensis MA-1T, and Dechloromonas denitrificans ATCC BAA-841T) were 26.5, 23.5, and 23.8 %. Growth occurs at 18?37°C on R2A medium in the presence of 0?0.5% NaCl (w/v) and at pH 5.5?8.0. The DNA G + C content of the genomic DNA was 62.9 mol%, and ubiquinone-8 (Q-8) was the major respiratory quinone. The major cellular fatty acids (> 5%) were of summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:0, C12:0, C10:0 3OH, summed feature 2 (C14:0 3OH/C16:1 iso I), and summed feature 8 (C18:1 ω7c/C18:1 ω6c). The polar lipids consisted of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), and diphosphatidylglycerol (DPG). Physiological and biochemical characteristics indicated that strain XY25T represents a novel species of the genus Dechloromonas, for which the name Dechloromonas hankyongensis sp. nov. is proposed. The type strain is XY25T(= KACC 22221T = LMG 32191T).
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KEYWORD
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Dechloromonas, polyphasic taxonomy, wetland, 16S rRNA gene sequence
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